Islet Cell Biology Core – Services
Provide rat, mouse and human islets from normal and diabetic sources.
The staff obtains the appropriate animals and using approved protocols, prepares islets and initiates cultures or other special handling as requested. They also supervise or teach the assays of hormone secretion that are part of the quality control protocols.
Maintain insulinomas and insulinoma cell lines for distribution
Dr. Steiner’s laboratory maintains a large number of insulinoma, neuroendocrine, and other relevant cell lines. The laboratory directly confers with Investigators and provides flasks of cells or frozen cells at particular passage numbers as requested, and from time to time test the viability and quality of specific lots. The laboratory maintains logs of cell lines available, storage and growth conditions, passage number, and quality control aspects, such as glucose-stimulated insulin secretion and mycoplasma monitoring. A partial listing of available material includes HIT, RINm5f, BTC3, BTC6, INS-1 (several kinds), MIN-6, HEK, COS-7, CHO, HeLa and SF9. A large number of mouse, rat, hamster and human insulinoma samples are also available.
Provide training in isolation and culture of pancreatic islets from mice, rats and humans.
A major function of the laboratory is to provide training in islet isolation, culture and biology. Interested faculty, fellows, and students are provided with hands-on training from experienced technicians so that they can gain first hand knowledge of these important skills.
Provide service and training in methods for studying insulin synthesis and secretion using a variety of methods.
Insulin biosynthesis has been a main focus of Dr. Steiner’s laboratory. A variety of approaches have been provided for studying islet hormone biosynthesis in great detail. Insulin secretion can be studied in a variety of ways. Both static and dynamic approaches to insulin secretion measurements are available. Direct and indirect, biophysical measures are also provided. These studies are carried out in close coordination with the requesting investigator.
Provide service and training in methods for immunohistochemical analysis of islets and insulin-secreting cells.
Investigators desiring these services consult with the Director and determine the appropriate reagents and conditions for particular studies. Histochemical studies are coordinated with the assistance of the Pathology section of the Animal Resource Center at the University of Chicago, the Electron Microscopy Core, and the Digital Imaging Core. Digital images are prepared for publication. Archival data storage of key images, reconstructions, and movie files are also maintained.
Provide service and training in calcium imaging and other biophysical methods for studying islet and beta-cell physiology
Investigators have available a number of techniques, including optical measurement of NAD(P)H, Rh123, TMRE, fura-2, fluo-3, MgGreen, FuraRed, and MgFura. Also included is detection of FRET (fluorescence resonance energy transfer) substrates and use of targeted GFP-based fusion proteins. The emphasis of the Core is on specialized live-cell techniques and dedicated islet perfusion systems. The Core also provides an interface to appropriate Divisional Cores, such as the Digital Imaging Core and Electron Microscopy Core of the Division of Biological Sciences. Through collaboration with Dr. Norbert Scherer in the Department of Chemistry, multiphoton imaging analysis is also available.