Islet Cell Biology Core – Objectives

The overall objective of this Core is to provide high quality islet and insulin–secreting cells to investigators together with basic and advanced methods to evaluate islet function. Specific objectives are:

  • Advice, service and training in the isolation of pancreatic islets from normal and diabetic rodents and humans
  • Provide insulinoma and other endocrine cell lines and advice on their use
  • Advice, service and training in the characterization of rodent and human pancreatic islets and beta cells
    • Proinsulin biosynthesis, processing and insulin secretion
    • Biophysical methods for studying islet and beta-cell function (e.g. calcium imaging, electrophysiology, total internal reflection fluorescent microscopy)
    • Biochemical methods for studying beta-cell function (e.g. phosphorylation, proliferation, apoptosis)
    • Immunohistochemical analysis of pancreatic islets
  • Advice, service and training on the use of adenovirus-based expression constructs to study protein function in beta cells and other cell types in vitro and in vivo